pENTR 2B Dual载体基本信息
| 载体名称: | pENTR 2B |
|---|---|
| 质粒类型: | Gateway系统载体；入门载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | -- |
| 克隆方法: | -- |
| 载体大小: | 3755 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | Kanamycin.html' target='_blank'>卡那霉素（Kanamycin） |
| 筛选标记: | 氯霉素 |
| 备注: | 克隆宿主Survival2T1，不建议使用常用的DH5alpha 和TOP10。 |
| 产品目录号: | #A10467 |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | -- |
Gateway® entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to c
reate a Gateway® entry clone. The resulting entry clone is ready for recombination with a destination vector
to create an expression clone.
The Gateway entry vectors offer the following:
• attL1 and attL2 sites for site-specific recombination of the entry clone with a Gateway® destination
vector to ensure cloning of the gene of interest in the correct orientation for expression
• Kozak consensus sequence for efficient translation initiation in eukaryotic systems
• Ribosome binding site for efficient translation initiation in prokaryotic systems (pENTR™ 1A Dual
Selection, pENTR™3C Dual Selection, and pENTR™11 Dual Selection vectors only)
• rrnB transcription termination sequences to prevent basal expression of the PCR product of interest in E. coli
• pUC origin for high-copy replication and maintenance of the plasmid in E. coli
• Kanamycin resistance gene for selection in E. coli
• The ccdB⁄chloramphenicol fusion gene located between the two attL sites for negative selection and
o Chloramphenicol selection in E. coli
• Kanamycin resistance gene for selection in E. coli