pLVX-Tet3G

产品信息
基本信息
质粒简介
质粒图谱
质粒序列

产品信息

产品货号	产品名称	产品规格	优惠价
XY0271	pLVX-Tet3G	20μl	¥请询价

使用说明

信裕质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产品的三个月内通知我司。收到质粒后请短暂离心，加入20μl ddH2O溶解质粒，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。如需获取其他详细信息请登录我司官网查询。

基本信息

启动子:	CMV
复制子:	pUC ori
终止子:	SV40 poly(A) signal
质粒分类:	病毒系列，慢病毒克隆载体
质粒大小:	9051bp
原核抗性:	Amp
克隆菌株:	Stbl3
培养条件:	37℃，有氧 LB
表达宿主:	哺乳细胞
诱导方式:	四环素诱导
5'测序引物:	CMV-F：CGCAAATGGGCGGTAGGCGTG
3'测序引物:	根据序列设计引物

质粒简介

plvx-tet3g是稳定表达对3G转录激活蛋白Tet调控载体，作为任何lenti-x™部分Tet对3G诱导表达系统。3G诱导表达系统的lenti-x TET允许病毒在各种各样的哺乳动物细胞中的你的兴趣基因的递送和诱导表达。表达的靶细胞Tet对3G的反式激活蛋白从plvx-tet3g和含有目的基因的控制下的一个ptre3g启动子将高水平表达你的基因，只有在存在强力霉素培养。

质粒图谱

质粒序列

LOCUS Exported 9051 bp ds-DNA circular SYN 29-AUG-2016

DEFINITION synthetic circular DNA

ACCESSION .

VERSION .

KEYWORDS pLVX-Tet3G

SOURCE synthetic DNA construct

ORGANISM synthetic DNA construct

REFERENCE 1 (bases 1 to 9051)

AUTHORS .

TITLE Direct Submission

JOURNAL Exported Thursday, September 8, 2016 from SnapGene Viewer 3.1.4

http://www.miaolingbio.com

FEATURES Location/Qualifiers

source 1..9051

/organism="synthetic DNA construct"

/mol_type="other DNA"

LTR 1..634

/note="3' LTR"

/note="3' long terminal repeat (LTR) from HIV-1"

misc_feature 681..806

/note="HIV-1 Psi"

/note="packaging signal of human immunodeficiency virus

type 1"

misc_feature 1303..1536

/note="RRE"

/note="The Rev response element (RRE) of HIV-1 allows for

Rev-dependent mRNA export from the nucleus to the

cytoplasm."

misc_feature 2028..2143

/note="cPPT/CTS"

/note="central polypurine tract and central termination

sequence of HIV-1 (lacking the first T)"

enhancer 2201..2504

/note="CMV enhancer"

/note="human cytomegalovirus immediate early enhancer"

promoter 2505..2708

/note="CMV promoter"

/note="human cytomegalovirus (CMV) immediate early

promoter"

CDS 2821..3567

/codon_start=1

/product="modified rtTA protein that binds tightly to

promoters containing the tet operator in the presence of

doxycycline"

/note="Tet-On(R) 3G"

/translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV

KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR

PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT

DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA

DALDDFDLDMLPADALDDFDLDMLPG"

misc_feature 3576..4148

/note="IRES"

/note="internal ribosome entry site (IRES) of the

encephalomyocarditis virus (EMCV)"

CDS 4162..4956

/codon_start=1

/gene="aph(3')-II (or nptII)"

/product="aminoglycoside phosphotransferase from Tn5"

/note="NeoR/KanR"

/note="confers resistance to neomycin, kanamycin, and G418

(Geneticin(R))"

/translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

misc_feature 4970..5558

/note="WPRE"

/note="woodchuck hepatitis virus posttranscriptional

regulatory element"

CDS complement(5441..5452)

/codon_start=1

/product="Factor Xa recognition and cleavage site"

/note="Factor Xa site"

/translation="IEGR"

LTR 5766..6399

/note="3' LTR"

/note="3' long terminal repeat (LTR) from HIV-1"

primer_bind complement(6528..6544)

/note="M13 rev"

/note="common sequencing primer, one of multiple similar

variants"

protein_bind 6552..6568

/bound_moiety="lac repressor encoded by lacI"

/note="lac operator"

/note="The lac repressor binds to the lac operator to

inhibit transcription in E. coli. This inhibition can be

relieved by adding lactose or

isopropyl-beta-D-thiogalactopyranoside (IPTG)."

promoter complement(6576..6606)

/note="lac promoter"

/note="promoter for the E. coli lac operon"

protein_bind 6621..6642

/bound_moiety="E. coli catabolite activator protein"

/note="CAP binding site"

/note="CAP binding activates transcription in the presence

of cAMP."

rep_origin complement(6930..7518)

/direction=LEFT

/note="ori"

/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

replication"

CDS complement(7689..8549)

/codon_start=1

/gene="bla"

/product="beta-lactamase"

/note="AmpR"

/note="confers resistance to ampicillin, carbenicillin, and

related antibiotics"

/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

LIKHW"

promoter complement(8550..8654)

/gene="bla"

/note="AmpR promoter"

polyA_signal 8702..8836

/note="SV40 poly(A) signal"

/note="SV40 polyadenylation signal"

ORIGIN

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca

61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac

121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca

181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg

241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag

301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg

361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat

421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga

481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct

541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc

601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag

661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg

721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga

781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg

841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg

901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct

961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat

1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca

1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc

1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag

1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc

1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg

1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc

1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc

1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct

1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa

1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca

1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt

1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt

1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta

1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt

1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct

1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga

1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag

2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac

2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg

2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa

2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg

2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt

2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg

2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc

2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc

2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca

2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta

2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa

2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc

2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgaggccacc

2821 atgtctagac tggacaagag caaagtcata aactctgctc tggaattact caatggagtc

2881 ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc tgggagttga gcagcctacc

2941 ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat cgagatgctg

3001 gacaggcatc atacccactc ctgccccctg gaaggcgagt catggcaaga ctttctgcgg

3061 aacaacgcca agtcataccg ctgtgctctc ctctcacatc gcgacggggc taaagtgcat

3121 ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg aaaatcagct cgcgttcctg

3181 tgtcagcaag gcttctccct ggagaacgca ctgtacgctc tgtccgccgt gggccacttt

3241 acactgggct gcgtattgga ggaacaggag catcaagtag caaaagagga aagagagaca

3301 cctaccaccg attctatgcc cccacttctg aaacaagcaa ttgagctgtt cgaccggcag

3361 ggagccgaac ctgccttcct tttcggcctg gaactaatca tatgtggcct ggagaaacag

3421 ctaaagtgcg aaagcggcgg gccgaccgac gcccttgacg attttgactt agacatgctc

3481 ccagccgatg cccttgacga ctttgacctt gatatgctgc ctgctgacgc tcttgacgat

3541 tttgaccttg acatgctccc cgggtaagga tcccgcccct ctccctcccc cccccctaac

3601 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc

3661 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg

3721 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg

3781 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc

3841 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa

3901 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa

3961 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta

4021 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg

4081 aggttaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac

4141 gatgataagc ttgccacaac catggctgaa caagatggat tgcacgcagg ttctccggcc

4201 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat

4261 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg

4321 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg

4381 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta

4441 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta

4501 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc

4561 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc

4621 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg

4681 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg

4741 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt

4801 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc

4861 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc

4921 atcgccttct atcgccttct tgacgagttc ttctgaacgc gtctggaaca atcaacctct

4981 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct

5041 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat

5101 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt

5161 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat

5221 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc

5281 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga

5341 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc

5401 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga

5461 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc

5521 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga

5581 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt

5641 gcctggctag aagcacaaga ggaggaggag gtgggttttt ccagtcacac ctcaggtacc

5701 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaagag

5761 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta

5821 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata

5881 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga

5941 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga

6001 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc

6061 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact

6121 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct

6181 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga

6241 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct

6301 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat

6361 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta

6421 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt

6481 gctagcgttt taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt

6541 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag

6601 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg

6661 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg

6721 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc

6781 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc

6841 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg

6901 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat

6961 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag

7021 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga

7081 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg

7141 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt

7201 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac

7261 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc

7321 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt

7381 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc

7441 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc

7501 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg

7561 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag

7621 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg

7681 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt

7741 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca

7801 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca

7861 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc

7921 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt

7981 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg

8041 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc

8101 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg

8161 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga

8221 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga

8281 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta

8341 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg

8401 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact

8461 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata

8521 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt

8581 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa

8641 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat

8701 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac

8761 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc

8821 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac

8881 cccataccct attaccactg ccaattacct agtggtttca tttactctaa acctgtgatt

8941 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag

9001 tagtttttaa agaaattgta tttgttaaat atgtactaca aacttagtag t