mRNA cap甲基转移酶抗体

规格：1mg/1ml

英文名： HCM

别名： RNMT; DKFZp686H1252; EC 2.1.1.56; hcm1p; hCMT1; HCMT1a; hCMT1c; hMet; hypothetical protein KIAA0396; KIAA0398; MCES_HUMAN; MET; mRNA (guanine 7)methyltransferase; mRNA (guanine N(7)) methyltransferase

分子量： 55kDa

储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce

克隆类型：Polyclonal

亚型：IgG

纯化方法：affinity purified by Protein A

免疫原：KLH conjugated synthetic peptide derived from human HCM

交叉反应：Human, Mouse, Rat, Chicken, Dog, Pig, Cow,

细胞定位：

mRNA cap甲基转移酶抗体产品介绍：background: RNMT (RNA (guanine-7-) methyltransferase) is a widely expressed nuclear protein that belongs to the mRNA cap methyltransferase family. It is responsible for catalyzing the final step in the attachment of the m7GpppN cap to the 5' end of mRNA. Capping of mRNA plays an important role in mRNA processing, stability and translation and is therefore important for efficient gene expression. There are three enzymatic steps in the generation of the mRNA cap. The first two steps are catalyzed by RNGTT (RNA guanylyltransferase and 5' phosphatase), and the third step is catalyzed by RNMT. More specifically, RNMT catalyzes the transfer of a methyl group from AdoMet (S-adenosylmethionine) to the GpppN end of the growing mRNA at the N-7 position, thereby producing AdoHyc (S-adenosylhomocysteine)mRNA cap甲基转移酶抗体 and m7GpppN terminated RNA. Two additional isoforms of RNMT exist due to alternative splicing events, namely hCMT1a and hCMT1b. Function: mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC. Subunit: Interacts with importin alpha, leading to stimulate both RNA-binding and methyltransferase activity. Interaction with importin alpha and beta is required for its nuclear localization, importin beta dissociating in response to RanGTP, allowing RNMT-importin alpha to bind RNA substrates. Interacts with elongating form of polymerase II and RNGTT. Interacts with RAM/FAM103A1, themRNA cap甲基转移酶抗体 interaction significantly enhances RNA-binding and cap methyltransferase activity. Subcellular Location: Nucleus. Tissue Specificity: Widely expressed. Similarity: Belongs to the class I-like SAM-binding methyltransferase superfamily. mRNA cap 0 methyltransferase family. Contains 1 mRNA cap 0 methyltransferase domain. Gene ID: 8731 Database links: Entrez Gene: 8731 Human Omim: 603514 Human SwissProt: O43148 Human Unigene: 592347 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

mRNA cap甲基转移酶抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.

研究领域：细胞生物  免疫学  染色质和核信号  表观遗传学  

储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

来源： Rabbit

外观： Lyophilized or Liquid