人类乳头瘤病毒筛查HPV screening仪器专业为人类乳头瘤病毒筛查HPV screening 应用而设计，人类乳头瘤病毒筛查HPV screening非常有助于早期发现HPV HR感染的风险而发现宫颈癌等癌症早期的症状。
人类乳头瘤病毒筛查HPV screening仪器具有PapilloCheck芯片微阵列，非常容易筛查鉴定24 个HPV类型，包括18种高风险人乳头瘤病毒和6种低风险病毒。
高风险人乳头瘤病毒 （HPV）: 16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 70, 73, 82.
低风险人乳头瘤病毒（HPV） : 6, 11, 40, 42, 43, 44.

市面上其它人乳头瘤病毒（HPV）测试系统只能将人乳头瘤病毒（HPV）分类成高或低风险组，但乳头瘤检测（PapilloCheck®）可以对24种 HPV同时进行检测，提高诊断质量。 PapilloCheck®保证高的特异性和敏感性。

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The test is based on the detection of E1 gene fragment of 24 HPV types.
The first step is the extraction of viral DNA from a cervical specimen. A fragment of 350pb of the E1 gene is amplified by PCR in presence of specific HPV primers. To avoid false-negative results, a fragment of house keeping gene ADAT1 (adenosine deaminase 1) is amplified in the same experiment.

The amplification products are hybridized for only 15 minutes at room temperature. Every chip has 12 arrays allowing simultaneous analysis of 12 samples. During hybridization, probes are labelled with fluorescent dyes. Chips are washed to eliminate unbound DNA and then analysed.

PapilloCheck^® features:

• DNA chip for specific identification of 18 high risk HPV and 6 low risk.
• First and second intention test for the cervical cancer screenings.
• Label CE (IVD). Recording made within the framwork of quantitative HPV detection on samples.

PapilloCheck^® kit content

• 5 slides with 12 tests each • Mastermix PCR • Hybridization buffer • Wash Buffer A • Wash Buffer B • CheckReport™ software with user guide

• Certified in-vitro diagnostic (IVD) for simultaneous genotyping of 24 different types of HPV.
• Controls integrated on the chip – Controls and patient in a single sample.
• High specificity and high sensibility.
• Automated scan, evaluation and HPV report generation.

Protocol: less than 4 h (including PCR )

1. Sample collection: Cervical cells, biopsy 30 min

2. DNA Extraction:  30 min

3. PCR: 120 min

4. Hybridization: 15 min at the ambient

5. Washes: 2 min

6. Scaning:  10 min