Affinity Chromatography and Chiral Separation LC C

The columns have the following features:

1) Rigid polymeric media enable high-performance analysis.

2) Chemically stable bonding groups (spacers) between ligands and substrates minimize detachment of ligands, ensuring highly reproducible analysis. Up to hundreds of runs are reproducible.

Product	Ligand	Particle size	Size	Part number
		(micron)	(mm) ID x Length
AFpak ACH-494	Choline oxydase, acetylcholine esterase	18	4.6 x 10	F7118964
AFpak ALC-894	Lentil lectin	18	8.0 x 50	F7113080
AFpak ALS-894	Lysine (LCA)	18	8.0 x 50	F7118962
AFpak ANA-894	NAD	18	8.0 x 50	F7113020
AFpak AOV-894	Ovomucoid	18	8.0 x 50	F7118958
AFpak APA-894	Protein A	18	8.0 x 50	F7118946
AFpak APB-894	Aminophenyl boronic acid	10	8.0 x 50	F7118950
AFpak APD-894	Procion red	18	8.0 x 50	F7113030
AFpak APE-894	Phosphorylethanolamine	18	8.0 x 50	F7113070
AFpak APG-894	Protein G	18	8.0 x 50	F7113050

Chiral Separation (ORpak CD)

Besides the positive and negative properties of optical rotation, optical isomers have the same physical and chemical characteristics. Optical separation columns are indispensable for direct analysis without derivatization. Shodex ORpak series incorporate packing material bonded by ligands that can identify many optical isomers.

ORpak series has the following ligands: cyclodextrin derivative (CD).

Because CD is used as the ligand, these columns are suitable for the analysis of optical isomers that make up an inclusion complex with CD. alpha-CD is used as the ligand in CDA, beta-CD is used as the ligand in CDB and CDBS and gamma-CD is used as the ligand in CDC. As a result, different columns can be used based on the size of the hydrophobic area of the optical isomer that is included. Packing materials in CDB and CDBS columns are different. Since CDBS is packed with silica gel, the peaks are sharp and many optical isomers can be analysed. Since CDB is packed with polymer-based gel, a wide range of separation conditions is possible, including alkaline separation. Since beta-CD identifies the most optical isomers within the CD's, CDB and CDBS are suitable for preliminary studies of optical isomer analysis. The CD series are suitable for the analysis of, not only optical isomers, but also structural isomers that have similar structure.

Product	Ligand	Particle size	Size	Part number
		(micron)	(mm) ID x Length
ORpak CDA-453 HQ	alpha-Cyclodextrin derivatives	6	4.6 x 150	F7146000
ORpak CDB-453 HQ	beta-Cyclodextrin derivatives	6	4.6 x 150	F7146001
ORpak CDC-453 HQ	gamma-Cyclodextrin derivatives	6	4.6 x 150	F7146002
ORpak CDBS-453	beta-Cyclodextrin derivatives	3	4.6 x 150	F7146003
ORpak CD-G		6	4.6 x 10	F6709250

Chiral Separation (ORpak CRX-853)

These are columns for separating optical isomers by ligand exchange using L-amino acid derivatives as the ligand. Eluent containing copper ions are used such as copper sulfate solutions. These columns can be used to analyze optical isomers of compounds which comprise a complex with copper, such as amino acids and acid hydroxyl groups, as well as their derivatives.

Product	Ligand	Particle size	Size	Part number
		(micron)	(mm) ID x Length
ORpak CRX-853	L-amino acid derivative	10	8.0 x 50	F7140040
ORpak CRX-G		6	4.6 x 10	F6709300

Chiral Separation (AFpak ABA-894)

Product	Ligand	Particle size	Size	Part number
		(micron)	(mm) ID x Length
AFpak ABA-894	Bovine serum albumin (BSA)	6	8.0 x 50	F7118960