糖原磷酸化酶M(PYGM)重组蛋白

Recombinant Glycogen Phosphorylase, Muscle (PYGM)

FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!

Organism species	Mus musculus (Mouse)
Product No.	KL848Mu01
Source	Prokaryotic expression
Host	E.coli
Purity	> 95%
UOM	50ug
Predicted Molecular Mass	22.0kDa
Concentration	n/a
Applications	SDS-PAGE; WB; ELISA; IP.
Endotoxin Level	<1.0EU per 1µg (determined by the LAL method)
Residues	Arg11~Gly187 with N-terminal His-Tag

Formulation

Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

糖原磷酸化酶M(PYGM)重组蛋白SEQUENCES

The sequence of the target protein is listed below.
RKQISVRGLA GVENVSELKK NFNRHLHFTL VKDRNVATPR DYYFALAHTV RDHLVGRWIR TQQHYYEKDP KRIYYLSLEF YMGRTLQNTM VNLALENACD EATYQLGLDM EELEEIEEDA GLGNGGLGRL AACFLDSMAT LGLAAYGYGI RYEFGIFNQK ICGGWQMEEA DDWLRYG

糖原磷酸化酶M(PYGM)重组蛋白USAGE

Reconstitute in sterile ddH2O.

糖原磷酸化酶M(PYGM)重组蛋白STORAGE AND STABILITY

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8^oC for one month. Aliquot and store at -80^oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37^oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

糖原磷酸化酶M(PYGM)重组蛋白About the MARKER (complimentary)

Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.

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