白细胞介素-28A蛋白rMuIFN-λ2 IL-28A

重组小鼠干扰素-λ2/白细胞介素-28A蛋白

Species	Murine
Accession	Q4VK74
GeneID	330496
Source	Escherichia coli.
Molecular Weight	重组小鼠干扰素-λ2/白细胞介素-28A蛋白Approximately 19.7 kDa, a single non-glycosylated polypeptide chain containing 174 amino acids.
Quantity	5µg/20µg/1000µg
AA Sequence	DPVPRATRLP VEAKDCHIAQ FKSLSPKELQ AFKKAKDAIE KRLLEKDMRC SSHLISRAWD LKQLQVQERP KALQAEVALT LKVWENMTDS ALATILGQPL HTLSHIHSQL QTCTQLQATA EPKPPSRRLS RWLHRLQEAQ SKETPGCLED SVTSNLFRLL TRDLKCVASG DQCV
Purity	> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity	重组小鼠干扰素-λ2/白细胞介素-28A蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by an anti-viral assay using human HepG2 cells infected with encephalomyocarditis is less than 3 ng/ml, corresponding to a specific activity of > 3.3 × 10⁵ IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4, with 5 % trehalose.
Endotoxin	Less than 0.1 EU/µg of rMuIFN-λ2/IL-28A as determined by LAL method.
Reconstitution	重组小鼠干扰素-λ2/白细胞介素-28A蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重组小鼠干扰素-λ2/白细胞介素-28A蛋白
SDS-PAGE