唾液酸结合Ig样凝集素1(SIGLEC1)多克隆抗体 

Polyclonal Antibody

To Sialic Acid Binding Ig Like Lectin 1 (SIGLEC1)

Organism Species: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

10th Edition (Revised in Jan, 2014)

[ PRODUCT INFORMATION ]

Immunogen: SIGLEC1, Human

Clonality: Polyclonal

Host: Rabbit

Immunoglobulin Type: IgG

Purification: Affinity Chromatography.唾液酸结合Ig样凝集素1(SIGLEC1)多克隆抗体

Applications: WB, ICC, IHC-P, IHC-F, ELISA

Concentration: 200µg/mL

UOM: 100µg

[ IMMUNOGEN INFORMATION ]

Immunogen: Recombinant SIGLEC1 (Gly34~Gly240) with N-terminal His-Tag

expressed in E.coli.

Accession No.: RPA966Hu01

[ ANTIBODY SPECIFITY ]

The antibody is a rabbit polyclonal antibody raised against SIGLEC1. It has been

selected for its ability to recognize SIGLEC1 in immunohistochemical staining and

western blotting.

[ APPLICATIONS ]

Western blotting: 1:50-400

Immunocytochemistry in formalin fixed cells: 1:50-500

Immunohistochemistry in formalin fixed frozen section: 1:50-500

Immunohistochemistry in paraffin section: 1:10-100

Enzyme-linked Immunosorbent Assay: 1:100-200

Optimal working dilutions must be determined by end user.唾液酸结合Ig样凝集素1(SIGLEC1)多克隆抗体

[ CONTENTS ]

Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,

50% glycerol.

[ QUALITY CONTROL ]

Content: The quality control contains recombinant SIGLEC1 (Gly34~Gly240)

disposed in loading buffer.

Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.

5uL per well when used in enhanced chemilumescent (ECL).

Note: The quality control is specifically manufactured as the positive control.

Not used for other purposes.

Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,

BPB 0.01%, NaN3 0.02%.

[ STORAGE ]

Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer

for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

[ IMAGES ]

Used in Western Blot, Sample:

唾液酸结合Ig样凝集素1(SIGLEC1)多克隆抗体Recombinant SIGLEC1, Human

Used in DAB staining on fromalin fixed

paraffin- embedded liver tissue

• 来源多抗制备
• 宿主兔
• 效价-
• Ig类型IgG
• 纯化方式抗原特异性亲和纯化
• 标记物无标记物
• 免疫原-
• 缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
• 性状唾液酸结合Ig样凝集素1(SIGLEC1)多克隆抗体液体
• 浓度200ug/ml
• 且适物种-
• 应用WB, ICC, IHC-P, IHC-F, ELISA