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细胞外基质金属蛋白酶诱导因子(EMMPRIN)多克隆抗体
Polyclonal Antibody
To Extracellular Matrix Metalloproteinase Inducer (EMMPRIN)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PRODUCT INFORMATION ]
Immunogen: EMMPRIN, Human
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.细胞外基质金属蛋白酶诱导因子(EMMPRIN)多克隆抗体
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200µg/mL
UOM: 100µg
[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant EMMPRIN (Val79~Gln280) expressed in E.coli. Accession No.: RPB540Hu01
Sequence: The target protein is fused with two N-terminal Tags, His-tag and
T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- VH IHATYHQHAA STISIDTLVE
EDTGTYECRA SNDPDRNHLT RAPRVKWVRA QAVVLVLEPG TVFTTVEDLG SKILLTCSLN
DSATEVTG HR WLK GGVV LK E DA LPG QK TEF KV DS DDQWGE YS CV F LPEPM
GTANIQLHGP PRVKAVKSSE HINEGETAML VCKSESVPPV TDWAWYKITD SEDKALMNGS
ESRFFVSSSQ
[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against EMMPRIN. It has been
selected for its ability to recognize EMMPRIN in immunohistochemical staining and
western blotting.细胞外基质金属蛋白酶诱导因子(EMMPRIN)多克隆抗体
[ APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
[ IMAGES ]
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant EMMPRIN (Val79~Gln280)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).细胞外基质金属蛋白酶诱导因子(EMMPRIN)多克隆抗体
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC to -80
oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Used in DAB staining on fromalin fixed
paraffin-embedded Liver tissue
Used in Western Blot, Sample:
Recombinant EMMPRIN, Human
- 来源多抗制备
- 宿主兔
- 效价-
- Ig类型IgG
- 纯化方式抗原特异性亲和纯化
- 标记物细胞外基质金属蛋白酶诱导因子(EMMPRIN)多克隆抗体无标记物
- 免疫原-
- 缓冲液成份磷酸盐缓冲液(pH7.4,含有0.02% NaN3和50%甘油)
- 性状液体
- 浓度200ug/ml
- 且适物种-
- 应用WB, ICC, IHC-P, IHC-F, ELISA
- 询价产品:细胞外基质金属蛋白酶诱导因子(EMMPRIN)多克隆抗体 EMMPRIN
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