NFS-5 C-1细胞

细胞类型： 其他细胞类型

是否是肿瘤细胞： 0

物种来源： 小鼠

数量： 大量

运输方式： 冻存运输

细胞形态： 淋巴样

品系： NFS/N

ATCC Number： CRL-1693™

相关疾病： 淋巴瘤

生长状态： 悬浮生长

Designations: NFS-5 C-1

Depositors: WJ Davidson, HC Morse

NFS-5 C-1细胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus

Morphology: lymphoblast

Source: Disease: lymphoma

Strain: NFS/N

Cell Type: pre-B lymphoblast;

Cellular Products: ecotropic retrovirus; mink cell focus forming retrovirus

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Antigen Expression: CD5 +; CD32 +; CD45R +

Comments: The NFS-5 C-1 cell line was derived from a lymphoma arising in a mouse inoculated with CAS- 2SM ecotropic murine leukemia virus.

The cells bear a pattern of markers consistent with identification as large pre-B lymphoblasts (Ly 17, CD32.

Ly 1, CD5.

Lyb 2, Ly 5(B220), CD45R).

They are negative for ThB, surface immunoglobulin (sIg) and Ia.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: Modified Dulbecco's medium with 0.002 mg/ml transferrin and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%

Temperature: 37.0°C

Subculturing: Medium Renewal: Every 2 to 3 days

NFS-5 C-1细胞Cultures can be maintained by addition or replacement of fresh medium. Maintain cell concentration between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: culture medium 95%; DMSO, 5%

Related Products: recommended serum:ATCC 30-2020

Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X

References: 22836: Davidson WF, et al. NFS-5 C-1细胞A unique series of lymphomas related to the Ly-1+ lineage of B lymphocyte differentiation. J. Immunol. 133: 744-753, 1984. PubMed: 6330201