Ro Vid细胞
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仪表展览网>产品库>普通细胞
价格: 面议
产品型号:Ro Vid
公司名称:上海沪震实业有限公司
地:上海松江
发布时间:2017-01-13
上海沪震实业有限公司
产品简介
Ro Vid细胞应如何避免细胞污染,细胞污染的种类可分成细菌、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之最好方法。Ro Vid细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。
产品详情

Ro Vid细胞

生长状态: 贴壁生长

数量: 大量

细胞形态: 成纤维样

年限: 53 years

运输方式: 冻存运输

ATCC Number: CRL-1308™

相关疾病: 着色性干皮病

器官来源: 皮肤

是否是肿瘤细胞: 0

物种来源: 人

Designations: Ro Vid

Ro Vid细胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast


Source: Organ: skin

Disease: xeroderma pigmentosum, presumed heterozygote

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Ro Vid细胞Please click here for information regarding the specific requirements for shipment to your location.

Age: 53 years

Gender: male

Comments: Ro Vid细胞The tissue donor was a presumed heterozygote for xeroderma pigmentosum, and was the father of Jo Vid (ATCC CRL-1307).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Remove spent medium, add fresh 0.25% trypsin for 3 to 5 minutes, remove trypsin and let the culture sit at 37C for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Ro Vid细胞Subculture every 5 to 7 days.

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