HCT-8 (HRT-18)细胞

是否是肿瘤细胞： 1

物种来源： 人

ATCC Number： CCL-244™

相关疾病： 其他疾病

细胞形态： 上皮样

运输方式： 冻存运输

生长状态： 贴壁生长

器官来源： 结肠

数量： 大量

年限： 67 years adult

Designations: HCT-8 [HRT-18]

Depositors: A Sobrero

HCT-8 (HRT-18)细胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: colon

Disease: ileocecal colorectal adenocarcinoma

Cellular Products: carcinoembryonic antigen (CEA) 0.5 ng/10 exp6 cells/10 days; alkaline phosphatase; keratin

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Applications: The cells are positive for keratin by immunoperoxidase staining.

Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 12

D13S317: 8,11

D16S539: 12,13

D5S818: 13

HCT-8 (HRT-18)细胞D7S820: 10,12,11.3

THO1: 7,9.3

TPOX: 8,11

vWA: 18,19

Isoenzymes: AK-1, 1

ES-D, 1-2

G6PD, B

GLO-I, 2

Me-2, 1

PGM1, 1

PGM3, 1

Age: 67 years adult

Gender: male

Comments: The HCT-8 line is identical to the HRT-18 cell line.

The cells are positive for keratin by immunoperoxidase staining.

Propagation: HCT-8 (HRT-18)细胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, rinse cell sheet twice with 0.25% trypsin, 0.02% EDTA in Hanks' BSS and incubate at 37C with the trypsin, EDTA for 10 to 15 minutes. Remove trypsin, EDTA, disperse cells in fresh medium, centrifuge, resuspend cells in medium and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2001

Recommended serum: ATCC 30-2040

0.25% (w/v) Trypsin ? 0.53mM EDTA in Hank?s BSS (w/o Ca++, Mg ++): ATCC 30-2101

Cell culture tested DMSO: ATCC 4-X

Derivative: ATCC CRL-11663

References: 1052: Tompkins WA, et al. Cultural and antigenic properties of newly established cell strains derived from adenocarcinomas of the human colon and rectum. J. Natl. HCT-8 (HRT-18)细胞Cancer Inst. 52: 1101-1110, 1974. PubMed: 4826581

26064: Nelson-Rees WA, et al. Distinctive banded marker chromosomes of human tumor cell lines. Int. J. Cancer 16: 74-82, 1975. PubMed: 1058173

32376: White LJ, et al. Attachment and entry of recombinant norwalk virus capsids to cultured human and animal cell lines. J. Virol. 70: 6589-6597, 1996. PubMed: 8794293