pET-SUMO载体基本信息
| 载体名称: | pET-SUMO |
|---|---|
| 质粒类型: | 大肠杆菌表达载体 |
| 高拷贝/低拷贝: | 低拷贝 |
| 启动子: | T7 和 lacO |
| 克隆方法: | TA Cloning |
| 载体大小: | 5643bp |
| 5' 测序引物及序列: | T7 Forward |
| 3' 测序引物及序列: | -- |
| 载体标签: | His Tag(6x)；SUMO Tag |
| 载体抗性: | Kanamycin.html' target='_blank'>卡那霉素 |
| 筛选标记: | -- |
| 备注: | 宿主菌株BL21(DE3)；IPTG诱导 |
| 产品目录号: | K300-01 |
| 稳定性: | -- |
| 组成型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
The Champion pET-SUMO Expression System produces the highest levels of soluble protein in E. coli. It utilizes a small ubiquitin-related modifier (SUMO) fusion, belonging to the growing family of ubiquitin-related proteins, to enhance the solubility of expressed fusion proteins. In contrast to ubiquitin, SUMO is involved in the stabilization and localization of proteins in vivo. After expression, the 11 kd SUMO moiety can be cleaved by the highly specific and active SUMO (ULP-1) protease at the carboxyl terminal, producing a native protein*. The Champion pET SUMO Protein and Peptide Expression System offers:
Greatly enhanced solubility with an N-terminal SUMO fusionHighly efficient cleavage- produces native protein of interest with SUMO (ULP-1) protease*Highly specific cleavage- eliminates the chance of your protein of interest being internally digested, regardless of its amino acid sequenceSignificantly increased stability with SUMO fusion-can be used for small peptide productionT7lac promoter for high-level protein expressionN-terminal 6xHis tag for protein detection and purification
参考文献
A novel hematopoietic granulin induces proliferation of goldfish (Carassius auratus L.) macrophages.
Hanington PC, Barreda DR, Belosevic M, J Biol Chem (2006) 281:9963-9970
Product usage: The granulin amplicon was cloned into the pET-SUMO TA expression vector (Invitrogen) and transformed into chemically competent TOP10 Escherichia coli (Invitrogen) according to the manufacturer's specifications. Plasmid DNA containing the granulin expression construct was transfor