pET-16b载体基本信息
| 别名: | pET16b, pet 16b |
|---|---|
| 质粒类型: | 大肠杆菌蛋白表达 |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5711bp |
| 5' 测序引物: | T7 |
| 5' 测序引物序列: | 5'-TAATACGACTCACTATAGGG-3' |
| 载体标签: | N-His, N-Factor Xa蛋白酶切位点 |
| 载体抗性: | Ampicillin |
| 备注: |
Hosts: E.coli. Related vectors: pBR322.
|
| 产品目录号: | 69662-3 |
| 稳定性: | 瞬时表达 Transient |
| 组成型: | 组成型 Constitutive |
| 病毒/非病毒: | 非病毒 |
| Feature Name | Start | End | |
|---|---|---|---|
| T7_terminator | 316 | 188 | |
| T7_Terminal_primer | 256 | 274 | |
| T7_transl_en_RBS | 419 | 403 | |
| lacO | 464 | 437 | |
| T7_promoter | 482 | 464 | |
| pBRrevBam_primer | 585 | 566 | |
| lacI | 860 | 1951 | |
| tet (576 - 1190) | 2010 | 2624 | |
| ROP | 3263 | 3454 | |
| pGEX_3_primer | 3470 | 3448 | |
| Ampicillin | 5503 | 4643 | |
| AmpR_promoter | 5573 | 5545 |
| ORF | Start | End | |
|---|---|---|---|
| ORF frame 1 | 619 | 1119 | |
| ORF frame 2 | 992 | 1951 | |
| ORF frame 1 | 2429 | 1734 | |
| ORF frame 3 | 2007 | 2624 | |
| ORF frame 2 | 5503 | 4643 |
| Enzyme Name | Cut |
|---|---|
| ClaI | 24 |
| HindIII | 29 |
| BamHI | 319 |
| XhoI | 324 |
| NdeI | 331 |
| NcoI | 392 |
| XbaI | 431 |
| BglII | 497 |
| BclI | 1233 |
| ApaI | 1430 |
| HpaI | 1725 |
| EagI | 2287 |
| NruI | 2322 |
| MscI | 2794 |
| PstI | 4961 |
| AatII | 5638 |
| EcoRI | 5709 |
The pET-16b vector (Cat. No. 69662-3) carries an N-terminal His•Tag® sequence followed by a Factor Xa site and three cloning sites. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below.
