收到小鼠脾细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

小鼠脾细胞The spleen stores and purifies erythrocytes, metabolizes hemoglobin, and recycles iron. The spleen also provides a critical function for the immune system by mounting a primary immune response to antigens in the blood and synthesizing antibodies [1]. Splenocytes are mononuclear cells derived from the spleen and include T-lymphocytes, B-lymphocytes, NK-cells, and NK T-cells. Primary mouse splenocytes (MS) can be used to isolate CD4+ T-cells, CD8+ T-cells, and CD45R+ B cells.

MS from ScienCell Research Laboratories are isolated from normal adult CD-1 mouse spleen. MS are depleted of splenic macrophages, cryopreserved directly after isolation, and delivered frozen. Each vial contains at least 10 million cells in 1 ml volume. MS are quality control tested for viability. MS are negative for mycoplasma, bacteria, yeast, and fungi. MS can be maintained for a limited period of time in culture using the conditions provided by ScienCell Research Laboratories and are not intended for long-term culture.

Catalog No. M5540

Country of Manufacture United States

Product Code MS

Size/Quantity 10 million cells in 1 ml volume

Product Use MS are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.

Shipping Info Dry ice.

References [1] Mebius R, Kraal G. (2005) "Structure and function of the spleen." Nat Rev Immunol 5(8): 606-616.