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结缔组织生长因子(CTGF)单克隆抗体
- 来源单抗制备
- 宿主小鼠
- 效价(克隆号)-
- Ig亚型IgG
- 纯化方式抗原特异性亲和纯化
- 标记物无标记物
- 免疫原-
- 缓冲液成份磷酸盐缓冲液(pH7.4,含有0.02% NaN3和50%甘油)
- 性状液体
- 浓度500ug/ml
- 且适物种-
-
应用WB, ICC, IHC-P, IHC-F, ELISA
结缔组织生长因子(CTGF)单克隆抗体特异性
该抗体是针对CTGF的小鼠单克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别CTGF。
用法
免疫印迹:1:100-400
免疫细胞化学:1:50-500
免疫组织化学(冰冻切片):1:50-500
免疫组织化学(石蜡切片):1:10-100
酶联免疫吸附试验:1:100-1:5000
最佳稀释倍数最终由用户决定。
储存
经常使用则4°C保存。-20°C保存不超过一年。避免反复冻融。
结缔组织生长因子(CTGF)单克隆抗体稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。结缔组织生长因子(CTGF)单克隆抗体
Monoclonal Antibody to Connective Tissue Growth Factor (CTGF)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PRODUCT INFORMATION ]
Immunogen: CTGF, Human
Clonality: Monoclonal
Clone number: B1
Host: Mouse结缔组织生长因子(CTGF)单克隆抗体
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 500µg/mL
UOM: 200µg Sample: Recombinant CTGF, Human
[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant CTGF (Glu248~Ala349) expressed in E.coli. Accession No.: RPA010Hu01
Sequence: The target protein is fused with N-terminal His-Tag and its sequence
is listed below.
MGHHHHHHSGSEF-ENI KKGKKCIRTP KISKPIKFEL SGCTSMKTYR AKFCGVCTDG
RCCTPHRTTT LPVEFKCPDG EVMKKNMMFI KTCACHYNCP GDNDIFESLY YRKMYGDMA[ ANTIBODY SPECIFITY ]
The antibody is a mouse monoclonal antibody raised against CTGF. It has been
selected for its ability to recognize CTGF in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
结缔组织生长因子(CTGF)单克隆抗体Optimal working dilutions must be determined by end user.
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC to -80
oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles
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