TXK酪氨酸激酶(TXK)重组蛋白

• 物种Mus musculus (Mouse，小鼠)
• 性状冻干粉内毒素水平<1.0EU/µg(LAL法测定)
• 亚细胞定位n/a
• 预测分子量29.7kDa
• 实际分子量-(差异分析请参阅说明书)
• 片段与标签His286~Gly509 (Accession # P42682) with N-terminal His Tag
• 缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
• 纯度> 95%
• 等电点-
• TXK酪氨酸激酶(TXK)重组蛋白应用SDS-PAGE; WB; ELISA; IP
  用法
  PBS或其它。
  储存
  避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
  稳定性
  热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
  
  TXK酪氨酸激酶(TXK)重组蛋白
  TXK Tyrosine Kinase (TXK)
  Organism Species: Mus musculus (Mouse)
  Instruction manual
  FOR IN VITRO USE AND RESEARCH USE ONLY
  NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
  9th Edition (Revised in Jul, 2013)
  TXK酪氨酸激酶(TXK)重组蛋白[ PROPERTIES ]
  Residues: His286~Gly509 (Accession # P42682),
  with two N-terminal Tags, His-tag and T7-tag. Host: E. coli
  Subcellular Location: Cytoplasm. Nucleus. Cell
  membrane; Peripheral membrane protein.
  Purity: >95%
  Endotoxin Level: <1.0EU per 1μg
  (determined by the LAL method).
  Formulation: Supplied as lyophilized form in PBS,
  pH7.4, containing 5% sucrose, 0.01% sarcosyl.
  Predicted isoelectric point: 7.2
  Predicted Molecular Mass: 29.7kDa
  Applications: SDS-PAGE; WB; ELISA; IP.
  (May be suitable for use in other assays to be determined by the end user.)
  TXK酪氨酸激酶(TXK)重组蛋白[ USAGE ]
  Reconstitute in sterile PBS, pH7.2-pH7.4.[ STORAGE AND STABILITY ]
  Storage: Avoid repeated freeze/thaw cycles.
  Store at 2-8oC for one month.
  Aliquot and store at -80oC for 12 months.
  Stability Test: The thermal stability is described by the loss rate of the target
  protein. The loss rate was determined by accelerated thermal degradation test,
  that is, incubate the protein at 37oC for 48h, and no obvious degradation and
  precipitation were observed. (Referring from China Biological Products Standard,
  which was calculated by the Arrhenius equation.) The loss of this protein is less
  than 5% within the expiration date under appropriate storage condition.
  [ SEQUENCES ]
  The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its
  sequence is listed below.
  MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF -HLGEW RAHIPVAIKA
  INEGSMSEED FIEEAKVMMK LSHSRLVQLY GVCIQQKPLY IVTEFMENGC LLDYLRERKG
  QLQKALLLSM CQDICEGMAY LERSCYIHRD LAARNCLVSS ACVVKISDFG MARYVLDDEY
  ISSSGAKFPV KWCPPEVFHF NKYSSKSDVW SFGVLMWEVF TEGKMPFENK SNLQVVEAIS
  QGFRLYRPHL APMTIYRVMY SCWHESPKG