应用SDS-PAGE; WB; ELISA; IP
生物素酰胺酶(BTD)重组蛋白用法
PBS或其它。
储存
避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
生物素酰胺酶(BTD)重组蛋白
Recombinant Biotinidase (BTD)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
生物素酰胺酶(BTD)重组蛋白[ PROPERTIES ]
Residues: Ala42~Ala271
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: P43251
Host: E. coli
Subcellular Location: Secreted.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 5.4
Predicted Molecular Mass: 30.1kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
生物素酰胺酶(BTD)重组蛋白[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
AHTGEESVA DHHEAEYYVA AVYEHPSILS LNPLALISRQ EALELMNQNL DIYEQQVMTA
AQKDVQIIVF PEDGIHGFNF TRTSIYPFLD FMPSPQVVRW NPCLEPHRFN DTEVLQRLSC
MAIRGDMFLV ANLGTKEPCH SSDPRCPKDG RYQFNTNVVF SNNGTLVDRY RKHNLYFEAA
FDVPLKVDLI TFDTPFAGRF GIFTCFDILF FDPAIRVLRD YKVKHVVYPT A