| 产品详细信息： |
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| 产品名称： |
国外直购甘露醇脱氢酶（荧光假单胞菌） |
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| 产品货号： |
wi59542 |
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| 产品资料： |
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外观：悬浮液
在水中的溶解性：易溶
pH值：6.0
气味：无
状态：液体
保质期：在冰箱5年或以上
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成分：
名称 比例
D -甘露醇脱氢酶（荧光假单胞菌） 1％
硫酸铵 45％
叠氮钠 0.02％
水 54％
Appearance Liquid suspension
Specific Gravity ~ 1.5
Solubility in Water Readily soluble
pH Value 6.0
Odour None
Form Liquid
Stability Stable in a refrigerator for five or more years
Ingredients Name CAS Proportion
D-Mannitol Dehydrogenase (P. fluorescens) 1%
Ammonium Sulphate 45%
Sodium Azide 0.02%
Water 54%
PROPERTIES
1. ELECTROPHORETIC PURITY
- Single band on SDS-gel electrophoresis (MW ~ 56,661)
- Single major band on isoelectric focusing (pI ~ 5.5)
2. SPECIFIC ACTIVITY
48.8 U/mg protein on D-Mannitol at pH 8.6 and 25°C.
One Unit of mannitol dehydrogenase is defined as the amount of enzyme required to
produce one mole of NADH from NAD+ under the following assay conditions:
Tris/HCl buffer, pH 8.6 64 mM
BSA 0.8 mg/mL
D-Mannitol 5.2 mM
NAD+ 2.3 mM
3. OTHER ACTIVITIES
SUBSTRATE SPECIFIC ACTIVITY (U/mg protein)
D-Mannitol 48.8
D-Arabitol 27.2
Sorbitol 0.95
Xylitol n.d
Glycerol n.d
n.d. = not detected
4. PHYSICOCHEMICAL PROPERTIES:
Recommended conditions of use are at pH 8.6 and up to 40°C.
5. STORAGE AND USE CONDITIONS/RECOMMENDATIONS:
The enzyme is supplied as an ammonium sulphate suspension and should be stored at
4°C. For assay, this enzyme should be diluted in 1 mg/mL BSA. Swirl to mix the
enzyme suspension immediately prior to use.
Mannitol dehydrogenase (P. fluorescens)
EC 1.1.1.67
from Pseudomonas fluorescens. This recombinant enzyme has been expressed in E. coli and purified by affinity chromatography. Electrophoretically homogenous. In 3.2 M ammonium sulphate. Specific activity:
48.8 U/mg protein on D-mannitol (pH 8.6, 40°C). Low Km, suitable for D-mannitol determination.
Contaminants: none detected.
Stable at 4°C for > 4 years.
Mannitol dehydrogenase (P. fluorescens)
EC 1.1.1.67
from Pseudomonas fluorescens. This recombinant enzyme has been expressed in E. coli and purified by affinity chromatography. Electrophoretically homogenous. In 3.2 M ammonium sulphate. Specific activity:
48.8 U/mg protein on D-mannitol (pH 8.6, 40°C). Low Km, suitable for D-mannitol determination.
Contaminants: none detected.
Stable at 4°C for > 4 years.
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