Thermo HyperSep Retain-CX 三聚氰胺处理专用SPE柱齐全

HYPERSEP Retain-CX 60MG/3ML SPE Column, PK/50
产品简介:
三聚氰胺前处理

HyperSep Retain-CX 三聚氰胺处理专用

#60107-303 RMB 1251.00

HYPERSEP Retain-CX 60MG/3ML SPE Column, PK/50

产品简介:

三聚氰胺前处理
HyperSep Retain-CX 60mg/3mL
前处理：取5g样品加入50mL 0.1%的三氯乙酸溶液中，振荡1分钟。加入2mL 2%醋酸铅溶液，超声20分钟，转移至10mL离心管中，以8000rpm离心10分钟。取3mL上清液。
活化：3mL甲醇，3mL去离子水
上样：以1-2mL/min的流速上样
淋洗：2mL去离子水，3mL甲醇，将小柱抽干
洗脱：5mL 5%稀氨水的甲醇溶液
浓缩：50℃氮气吹干、2mL 20%甲醇水溶液再溶解

Generic Procedure for HyperSep Retain-CX:

1. Conditioning: Methanol followed by de-ionised water

2. Sample Loading: Load sample at a rate of 1-2mL/min.

3. Washing: Methanol / de-ionised water （5/95, v/v）

4. Elution: Organic solvent such as methanol or acetonitrile

Application Example – Melamine in Animal Feeds

Part Number: 60107-303

Phase: HyperSep Retain-CX

Volume: 3 mL

Bed Weight: 60 mg

Sample Pretreatment: To 5 g of animal feed, add 50 mL of 0.1% trichloroacetic acid aqueous solution. Vortex for 1 min, then add 2 mL of 2% lead acetate aqueous solution. Sonicate for 20 mins, then transfer a portion of the mixture to a 10 mL Centrifuge tube. Centrifuge at 8000 rpm for 10 mins.

Conditioning: 3 mL methanol followed by 3 mL deionized water

Application: Load 3 mL sample at 1-2 mL/min

Washing: 3 mL water followed by 3 mL methanol. Purge column to dryness

Elution: 5 mL ammonia in methanol （5:95, v/v）. Dry sample under nitrogen stream. Reconstitute with 20% methanol aqueous solution.