Column:

Polysulfonix-SCX, 120 Å, 5 µm, 4.6 x 250 mm

Mobile phase:

A : B = 62 : 38 ( v/v )
A: ACN;    B:Phosphoric acid sodium(2 →1000, Adjust pH 3.8 with Ammonia solution)                       

Flow rate:

0.5 mL/min

Temperature:

40 ^oC

Detection:

UV 215 nm

Injection volume:

10 µL

Sample:

1.  Arecoline hydrobromide (0.0995 mg/mL)