低温免疫组化钻石刀 Cryo Immuno Diamond Knife

第一款带有钻石台面的低温钻石刀，保证可以得到免疫组织化学所需的最好切片。

因为有钻石台面（见图示），所以正确拿取切片变得很容易；可以用取片器直接从钻石台面收集切片。

35° 的切削角度大大降低了切削过程中刀刃对样本的机械挤压，因此对于有蔗糖保护的样本更能较好的保持原有的结构。

切片厚度：30nm－1um

刀刃尺寸：2.0mm－3.0mm

产品选购：

货号	产品名称	角度	长度
DCIMM3520	Cryo Immuno Diamond Knife	35°	2.0mm
DCIMM3530	Cryo Immuno Diamond Knife	35°	3.0mm

利用此款钻石刀得到的切片图片：

Fusion of late endosome/lyosome with the Mycobacteria tuberculosis phagosome. Aldehyde fixed cryo-section labeled with anti- CD63 and protein-A gold 10nm. Scale bar = 100nm. Nicole van der Wel and Peter J. Peters, Netherlands Cancer Institute, Amsterdam.

参考资料：

A. Al-Amoudi, D. Studer and J. Dubochet: Cutting artefacts and cutting process in vitreous sections for cryo-electron microscopy. Journal of Structural Biology 150, pp. 109-121, 2005.

W. Liu, H. J. Geuze, J. W. Slot: Improving structural integrity of cryosections for immunogold labeling Histochemistry and Cell Biohgy, Vol. 106, PP. 41-55, 1996.

M. Michel, H. Gnagi and M. Muller: Diamonds are a cryosectioner's best friend. Journal of Microscopy, Vol 166, Pt 1, 43-56, 1992.

P. J. Peters: Cryo-lmmunogold Electron Microscopy, In Current Protocols in Cell Biology {J.S. Bonifacino, M. Dasso, J.B. Harford, J. Lippincott-Schwartz and K.M. Yamada, eds) pp. 4.7.1-4.7.12, 1999. Jonn Wiley & Sons, New York.

K. Richter: Cutting artifacts on ultrathin cryosections of biological bulk specimens. Micron, Vol. 25, No. 4, pp. 297-308. 1994.

P. Zhang, E. Bos, J. Heymann, H. Gnaegi, M. Kessel, P.J. Peters, S. Subramaniam: Direct visualisation of receptor arrays in frozen-hydrated sections and plungefrozen specimens of E.coli engineered to overproduce the chemotaxis receptor Tsr. Journal of Microscopy, Vol. 216, Pt 1, pp. 76-83, 2004.