Bioprinting--现货 011-96WK 012-384WK

n3dbio 磁力3D生物打印产品 Magnetic 3D Bioprinting

1. 种类: 96孔板/384孔板

2. 细胞打印成3D微球

3. 可快速实现微球打印（15分钟到数小时时间内）

4. 3D微球状态可达数天

5. 可用于构建多细胞肿瘤微球

订购信息 ：

一、货号：011-96WK ，96孔板生物打印试剂盒(96-well Bioprinting Kit)

试剂盒内含有：

1.600ul NanoShuttle^TM-PL（3管）

2.96孔磁力器（1个）

3.超低吸附96孔板（2块）

二、货号：012-384WK，384孔板生物打印试剂盒（384-well BioPrinting Kit）

试剂盒内含有：

1.600ul NanoShuttle^TM-PL（2管）

2.384孔磁力器（1个）

3.超低吸附384孔板（2块）

英文介绍

Rapidly print spheroids within hours!

Biocompatible nanoparticles² to magnetize cells
3D spheroids mimicking native tissue environments
Rapid printing of spheroids (15 min to a few hours)¹
Magnetized spheroids that are easy to handle/retrieve
No special equipment or media needed

Magnetic 3D Bioprinting

While other spheroid systems can mimic native cellular environments, they take a long time to form and are difficult to handle/retrieve. Magnetic 3D bioprinting addresses these issues by utilizing n3D’s core technology, magnetizing cells with NanoShuttle (NS) to print spheroids. In contrast to magnetic levitation using the Bio-Assembler, in magnetic 3D bioprinting, cells incubated with NS overnight are printed into spheroids by placing a 96-well plate full of magnetized cells atop a drive of magnets.¹ The magnets below the well aggregate the cells using mild magnetic forces to form a spheroid at the bottom of the well. Afteronly 15 min to a few hours, the plate of spheroids can be removed from the magnets and cultured long-term. NanoShuttle does not interfere with fluorescence or other biochemical assays, so the spheroid can be assayed in any number of ways.

Viability of PC3 spheroids of various sizes as measured by (left) the Real-time CellTiter-Glo assay (Promega, Madison, WI) and (right) live/dead staining

In magnetizing spheroids, adding and removing solutions is made easy by the use of magnetic forces to hold down spheroids during aspiration, limiting spheroid loss. Spheroids can also be picked up and transferred between vessels using magnetic tools such as the MagPen^TM. Magnetic forces can also be used to create co-cultures with fine spatial organization.²

Co-culture of primary human tracheal smooth muscle cells (green) and A549 human alveolar epithelial lung adenocarcinoma cells (red) at varying ratios

Magnetic 3D bioprinting is a rapid and effective tool to print spheroids that are representative of native cellular environments and easy to handle.

Products

	Product	Includes	Part #	Manual	Click to Buy!
	96-well Bioprinting Kit	600-μL Vials of NanoShuttle^TM-PL (3) 96-Well Spheroid Drive (1) 96-Well Holding Drive (1) Cell-Repellent 96-Well Plates (2)	011-96WK		现货询价
	384-well Bioprinting Kit	600-μL Vials of NanoShuttle^TM-PL (2) 384-Well Spheroid Drive (1) 384-Well Holding Drive (1) Cell-Repellent 384-Well Plates (2)	012-384WK		现货询价

*US prices only. Ask your local distributor for prices in your region.
Contact us for additional parts.

References

1. Timm, D. M. et al. A high-throughput three-dimensional cell migration assay for toxicity screening with mobile device-based macroscopic image analysis. Sci. Rep. 3, 3000 (2013)
2. Tseng, H. et al. Assembly of a three-dimensional multitype bronchiole coculture model using magnetic levitation. Tissue Eng. Part C. Methods 19, 665–75 (2013).