Concentration
5u/μl 
5 ' . . . A↓C C G G T . . . 3 '
3 ' . . . T G G C C↑A . . . 5 '

Storage Buffer
10mM Tris-HCl (pH 7.5), 250mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 100μg/ml BSA and 50% glycerol.

Unit Definition
5u (1μl) is defined as the amount of enzyme that is required to digest 1μg of DNA within 15 minutes at 37˚C in 20μl or 50μl of assay buffer.

Thermal Inactivation
65˚C for 20 minutes.

Quality Control Assays
Ligation/ Recutting Assay
After 5-fold overdigestion with AsiG I, 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of AsiG I for 16 hours at 37°C.

Ordering Information

Downloads
Manual
AsiG I (Age I)