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白细胞激活黏附因子(ALCAM)检测试剂盒
ELISA Kit for Activated Leukocyte Cell Adhesion Molecule (ALCAM)
Organism species | Homo sapiens (Human) |
Product No. | YBA002Hu |
Sample type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Format | 96T |
Assay length | 4.5 hours |
Detection range | 0.156-10ng/mL The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL |
Sensitivity | The minimum detectable dose of this kit is typically less than 0.059ng/mL. |
白细胞激活黏附因子(ALCAM)检测试剂盒
Recovery
白细胞激活黏附因子(ALCAM)检测试剂盒
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 92-101 | 95 |
EDTA plasma(n=5) | 89-104 | 98 |
heparin plasma(n=5) | 86-104 | 95 |
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 91-98% | 82-96% | 78-104% | 83-92% |
EDTA plasma(n=5) | 93-105% | 89-101% | 88-97% | 80-93% |
heparin plasma(n=5) | 90-103% | 96-105% | 97-105% | 96-105% |
Stability
白细胞激活黏附因子(ALCAM)检测试剂盒
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Test principle
白细胞激活黏附因子(ALCAM)检测试剂盒
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